https://wiki.opensourceecology.org/index.php?action=history&feed=atom&title=Heat_Shock_%28Gene_Insertion_Technique%29 2026-04-20T23:15:51Z Revision history for this page on the wiki MediaWiki 1.39.13 https://wiki.opensourceecology.org/index.php?title=Heat_Shock_(Gene_Insertion_Technique)&diff=209558&oldid=prev 2020-01-25T00:04:24Z

<p>Created Page + Added Basic Sections/Info</p> <p><b>New page</b></p><div>=Basics=<br /> *One of the simplest [[Gene Insertion Techniques]] for [[Gene Editing]] along with [[Electroporation]] and [[Gene Gun]]s<br /> *Uses Rapid Thermal Cycling of a mix of Microorganism, [[Growth Media]] , DNA to be Inserted, and [[Calcium Chloride]]<br /> *All that is really needed is the microorganism, the dna one wants to insert, wetware, a heating block or bath, and a lab refrigerator, freezer, or cooling bath<br /> <br /> =Process=<br /> *[https://www.youtube.com/watch?v=F0_q-fD_lyU A Video by the youtube channel &quot;The Thought Emporium&quot; ] This video also goes over basic culture growth, and media prep steps which can be skipped if understood<br /> <br /> =See Also=<br /> *<br /> <br /> =Useful Links=<br /> *[https://en.wikipedia.org/wiki/Heat_shock_response The Wikipedia Page on the Heat Shock Response]<br /> *[https://en.wikipedia.org/wiki/Gene_delivery#Heat_shock The Wikipedia Page on The Heat Shock Gene Insertion Technique]</div>

Eric