Microfluidics - Revision history

Rasmus: added abstract from paper in Nature Biotech / moved link

2017-06-10T21:29:39Z

added abstract from paper in Nature Biotech / moved link

← Older revision		Revision as of 21:29, 10 June 2017
Line 65:		Line 65:

	Harnessing recent advances in synthetic biology and paper microfluidics, cell-free paper-based diagnostics offer a platform for low cost, in-field tests with a very wide range of possible specificities. Synthetic gene networks can be designed to generate quantifiable outputs, such as chromoproteins, in the presence of specific input signals like heavy metals or viral RNA sequences. These are freeze-dried onto paper, along with the cellular machinery used for gene transcription and translation. When rehydrated, rapid determination of the presence/absence of a substance of interest can be made. With a simple visible readout, little or no laboratory experience or infrastructure is required.		Harnessing recent advances in synthetic biology and paper microfluidics, cell-free paper-based diagnostics offer a platform for low cost, in-field tests with a very wide range of possible specificities. Synthetic gene networks can be designed to generate quantifiable outputs, such as chromoproteins, in the presence of specific input signals like heavy metals or viral RNA sequences. These are freeze-dried onto paper, along with the cellular machinery used for gene transcription and translation. When rehydrated, rapid determination of the presence/absence of a substance of interest can be made. With a simple visible readout, little or no laboratory experience or infrastructure is required.

			==Metafluidics==
			Abstract from 2017 paper in ''Nature Biotech'' (open access): [http://www.nature.com/nbt/journal/v35/n6/full/nbt.3873.html '''"Open-source, community-driven microfluidics with Metafluidics"''']

			Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. We present [https://metafluidics.org/ Metafluidics], an open-source, community-driven repository that hosts digital design files, assembly specifications, and open-source software to enable users to build, configure, and operate a microfluidic device. We use Metafluidics to share designs and fabrication instructions for both a microfluidic ring-mixer device and a 32-channel tabletop microfluidic controller. This device and controller are applied to build genetic circuits using standard DNA assembly methods including ligation, Gateway, Gibson, and Golden Gate. Metafluidics is intended to enable a broad community of engineers, DIY enthusiasts, and other nontraditional participants with limited fabrication skills to contribute to microfluidic research.

	==Further Reading==		==Further Reading==
Line 71:		Line 76:
	* [http://www.dfa.org/ Diagnostics For All]		* [http://www.dfa.org/ Diagnostics For All]
	* [http://www.daktaridx.com/ Daktari]		* [http://www.daktaridx.com/ Daktari]
	* Nature Biotech (open access): [http://www.nature.com/nbt/journal/v35/n6/full/nbt.3873.html "Open-source, community-driven microfluidics with Metafluidics"]

Rasmus: added another image

2017-06-10T21:23:04Z

added another image

← Older revision		Revision as of 21:23, 10 June 2017
Line 1:		Line 1:
	{{Category=Health}}		{{Category=Health}}
	{{Category=Pests and weeds}}		{{Category=Pests and weeds}}

	[[File:Microfluidic01.jpg\|500px\|thumb\|right\|Microfluidic Chip]]		[[File:Microfluidic01.jpg\|500px\|thumb\|right\|Microfluidic Chip]]

	[[File:Microfluidic Chip iX-factory.jpg\|500px\|thumb\|right\|Microfluidic Chip fabricated in Glass. Channels are 50µm deep and 150µm wide.]]		[[File:Microfluidic Chip iX-factory.jpg\|500px\|thumb\|right\|Microfluidic Chip fabricated in Glass. Channels are 50µm deep and 150µm wide.]]

			[[File:MicrofluidicDevice.png\|500px\|thumb\|right\|A schematic of fluid flowing through a microfluidic device by capillary action.]]

	[http://en.wikipedia.org/wiki/Microfluidics Microfluidics] refers to a set of technologies that control the flow of minute amounts of liquids or gases — typically measured in nano- and picoliters — in a miniaturized system.		[http://en.wikipedia.org/wiki/Microfluidics Microfluidics] refers to a set of technologies that control the flow of minute amounts of liquids or gases — typically measured in nano- and picoliters — in a miniaturized system.

Rasmus: added another image

2017-06-10T21:16:38Z

added another image

← Older revision		Revision as of 21:16, 10 June 2017
Line 1:		Line 1:
	{{Category=Health}}		{{Category=Health}}
	{{Category=Pests and weeds}}		{{Category=Pests and weeds}}
	[[File:Microfluidic01.jpg\|~~400px~~\|thumb\|right\|Microfluidic Chip]]		[[File:Microfluidic01.jpg\|500px\|thumb\|right\|Microfluidic Chip]]

			[[File:Microfluidic Chip iX-factory.jpg\|500px\|thumb\|right\|Microfluidic Chip fabricated in Glass. Channels are 50µm deep and 150µm wide.]]

	[http://en.wikipedia.org/wiki/Microfluidics Microfluidics] refers to a set of technologies that control the flow of minute amounts of liquids or gases — typically measured in nano- and picoliters — in a miniaturized system.		[http://en.wikipedia.org/wiki/Microfluidics Microfluidics] refers to a set of technologies that control the flow of minute amounts of liquids or gases — typically measured in nano- and picoliters — in a miniaturized system.

Rasmus: /* Further Reading */ added link to new paper

2017-06-10T21:04:40Z

Further Reading: added link to new paper

← Older revision		Revision as of 21:04, 10 June 2017
Line 66:		Line 66:
	* [http://www.dfa.org/ Diagnostics For All]		* [http://www.dfa.org/ Diagnostics For All]
	* [http://www.daktaridx.com/ Daktari]		* [http://www.daktaridx.com/ Daktari]
			* Nature Biotech (open access): [http://www.nature.com/nbt/journal/v35/n6/full/nbt.3873.html "Open-source, community-driven microfluidics with Metafluidics"]

Rasmus: inserted section: ==OpenDiagnostics==

2017-05-16T14:45:19Z

inserted section: ==OpenDiagnostics==

← Older revision		Revision as of 14:45, 16 May 2017
Line 55:		Line 55:
	<!--copy and paste--><object width="446" height="326"><param name="movie" value="http://video.ted.com/assets/player/swf/EmbedPlayer.swf"></param><param name="allowFullScreen" value="true" /><param name="allowScriptAccess" value="always"/><param name="wmode" value="transparent"></param><param name="bgColor" value="#ffffff"></param> <param name="flashvars" value="vu=http://video.ted.com/talks/dynamic/GeorgeWhitesides_2009X-medium.flv&su=http://images.ted.com/images/ted/tedindex/embed-posters/GeorgeWhitesides-2009X.embed_thumbnail.jpg&vw=432&vh=240&ap=0&ti=760&introDuration=15330&adDuration=4000&postAdDuration=830&adKeys=talk=george_whitesides_a_lab_the_size_of_a_postage_stamp;year=2009;theme=what_s_next_in_tech;theme=design_like_you_give_a_damn;theme=rethinking_poverty;theme=a_taste_of_tedx;theme=tales_of_invention;event=TEDxBoston+2009;&preAdTag=tconf.ted/embed;tile=1;sz=512x288;" /><embed src="http://video.ted.com/assets/player/swf/EmbedPlayer.swf" pluginspace="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" wmode="transparent" bgColor="#ffffff" width="446" height="326" allowFullScreen="true" allowScriptAccess="always" flashvars="vu=http://video.ted.com/talks/dynamic/GeorgeWhitesides_2009X-medium.flv&su=http://images.ted.com/images/ted/tedindex/embed-posters/GeorgeWhitesides-2009X.embed_thumbnail.jpg&vw=432&vh=240&ap=0&ti=760&introDuration=15330&adDuration=4000&postAdDuration=830&adKeys=talk=george_whitesides_a_lab_the_size_of_a_postage_stamp;year=2009;theme=what_s_next_in_tech;theme=design_like_you_give_a_damn;theme=rethinking_poverty;theme=a_taste_of_tedx;theme=tales_of_invention;event=TEDxBoston+2009;"></embed></object>		<!--copy and paste--><object width="446" height="326"><param name="movie" value="http://video.ted.com/assets/player/swf/EmbedPlayer.swf"></param><param name="allowFullScreen" value="true" /><param name="allowScriptAccess" value="always"/><param name="wmode" value="transparent"></param><param name="bgColor" value="#ffffff"></param> <param name="flashvars" value="vu=http://video.ted.com/talks/dynamic/GeorgeWhitesides_2009X-medium.flv&su=http://images.ted.com/images/ted/tedindex/embed-posters/GeorgeWhitesides-2009X.embed_thumbnail.jpg&vw=432&vh=240&ap=0&ti=760&introDuration=15330&adDuration=4000&postAdDuration=830&adKeys=talk=george_whitesides_a_lab_the_size_of_a_postage_stamp;year=2009;theme=what_s_next_in_tech;theme=design_like_you_give_a_damn;theme=rethinking_poverty;theme=a_taste_of_tedx;theme=tales_of_invention;event=TEDxBoston+2009;&preAdTag=tconf.ted/embed;tile=1;sz=512x288;" /><embed src="http://video.ted.com/assets/player/swf/EmbedPlayer.swf" pluginspace="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" wmode="transparent" bgColor="#ffffff" width="446" height="326" allowFullScreen="true" allowScriptAccess="always" flashvars="vu=http://video.ted.com/talks/dynamic/GeorgeWhitesides_2009X-medium.flv&su=http://images.ted.com/images/ted/tedindex/embed-posters/GeorgeWhitesides-2009X.embed_thumbnail.jpg&vw=432&vh=240&ap=0&ti=760&introDuration=15330&adDuration=4000&postAdDuration=830&adKeys=talk=george_whitesides_a_lab_the_size_of_a_postage_stamp;year=2009;theme=what_s_next_in_tech;theme=design_like_you_give_a_damn;theme=rethinking_poverty;theme=a_taste_of_tedx;theme=tales_of_invention;event=TEDxBoston+2009;"></embed></object>
	</html>		</html>

			==OpenDiagnostics==
			[http://www.open-diagnostics.org/ OpenDiagnostics] are working to develop and promote a new type of open source, paper-based diagnostic. Proof-of-concept for similar tests has already been demonstrated for detecting Zika and Ebola viruses. Since they are low cost, thermally stable, and easy to use, these strips are well suited to bring diagnostic capabilities to the Global South – across areas spanning healthcare, agriculture and environmental monitoring.

			Harnessing recent advances in synthetic biology and paper microfluidics, cell-free paper-based diagnostics offer a platform for low cost, in-field tests with a very wide range of possible specificities. Synthetic gene networks can be designed to generate quantifiable outputs, such as chromoproteins, in the presence of specific input signals like heavy metals or viral RNA sequences. These are freeze-dried onto paper, along with the cellular machinery used for gene transcription and translation. When rehydrated, rapid determination of the presence/absence of a substance of interest can be made. With a simple visible readout, little or no laboratory experience or infrastructure is required.

	==Further Reading==		==Further Reading==

Conor: typo

2011-02-22T21:56:26Z

typo

← Older revision		Revision as of 21:56, 22 February 2011
Line 22:		Line 22:
	===DIY microfluidics methods===		===DIY microfluidics methods===
	*How to make a microfluidic chip using double-sided sellotape, glass slides and a scalpel: [http://www.rsc.org/Publishing/Journals/lc/Chips_and_Tips/Rapid_prototype.asp]. This requires inlet and outlet holes in the slide; perhaps these could be made with a [[Laser cutter\|laser cutter]]?		*How to make a microfluidic chip using double-sided sellotape, glass slides and a scalpel: [http://www.rsc.org/Publishing/Journals/lc/Chips_and_Tips/Rapid_prototype.asp]. This requires inlet and outlet holes in the slide; perhaps these could be made with a [[Laser cutter\|laser cutter]]?
	*Complex 3D microfluidic devices made with alternating layers of paper and double-sided tape: [http://www.pnas.org/content/105/50/19606.full]. The tape was cut using a laser cutter. The paper was treated with ~~photoresis~~ (a light-sensitive polymer) and exposed to UV light when masked with a transparency with a pattern printed onto it. Cost of fabricating a chip = $0.03.		*Complex 3D microfluidic devices made with alternating layers of paper and double-sided tape: [http://www.pnas.org/content/105/50/19606.full]. The tape was cut using a laser cutter. The paper was treated with photoresist (a light-sensitive polymer) and exposed to UV light when masked with a transparency with a pattern printed onto it. Cost of fabricating a chip = $0.03.
	* [http://www.purdue.edu/newsroom/research/2011/110125ZiaiePaper.html Disposable microfluidic devices created using regular wax paper]		* [http://www.purdue.edu/newsroom/research/2011/110125ZiaiePaper.html Disposable microfluidic devices created using regular wax paper]
	* [http://www.rsc.org/Publishing/ChemTech/Volume/2008/01/Shrinky-Dink_microfluidics.asp Shrinky Dink® microfluidics] - academic paper [http://shrink.eng.uci.edu/papers/2008_Grimes.pdf here]		* [http://www.rsc.org/Publishing/ChemTech/Volume/2008/01/Shrinky-Dink_microfluidics.asp Shrinky Dink® microfluidics] - academic paper [http://shrink.eng.uci.edu/papers/2008_Grimes.pdf here]

	===Channel designs===		===Channel designs===

Conor: /* Channel designs */

2011-02-22T17:43:11Z

Channel designs

← Older revision		Revision as of 17:43, 22 February 2011
Line 28:		Line 28:
	===Channel designs===		===Channel designs===
	Are there online repositories of channel designs for different purposes?		Are there online repositories of channel designs for different purposes?

			[http://groups.csail.mit.edu/cag/micado/index.html Micado] is open-source software for designing microfluidic chips.

	==Materials and Equipment Used==		==Materials and Equipment Used==

Conor at 16:57, 22 February 2011

2011-02-22T16:57:32Z

← Older revision		Revision as of 16:57, 22 February 2011
Line 17:		Line 17:
	*and many more.		*and many more.

	We are interested in very cheap, open-source ways of making microfluidic chips.		==Microfluidics in Open Source Ecology==
			We are interested in very cheap, open-source ways of making microfluidic chips. There are plenty of groups working on this; it's a matter of gathering the information.

			===DIY microfluidics methods===
			*How to make a microfluidic chip using double-sided sellotape, glass slides and a scalpel: [http://www.rsc.org/Publishing/Journals/lc/Chips_and_Tips/Rapid_prototype.asp]. This requires inlet and outlet holes in the slide; perhaps these could be made with a [[Laser cutter\|laser cutter]]?
			*Complex 3D microfluidic devices made with alternating layers of paper and double-sided tape: [http://www.pnas.org/content/105/50/19606.full]. The tape was cut using a laser cutter. The paper was treated with photoresis (a light-sensitive polymer) and exposed to UV light when masked with a transparency with a pattern printed onto it. Cost of fabricating a chip = $0.03.
			* [http://www.purdue.edu/newsroom/research/2011/110125ZiaiePaper.html Disposable microfluidic devices created using regular wax paper]
			* [http://www.rsc.org/Publishing/ChemTech/Volume/2008/01/Shrinky-Dink_microfluidics.asp Shrinky Dink® microfluidics] - academic paper [http://shrink.eng.uci.edu/papers/2008_Grimes.pdf here]

			===Channel designs===
			Are there online repositories of channel designs for different purposes?

	==Materials and Equipment Used==		==Materials and Equipment Used==
Line 45:		Line 55:

	==Further Reading==		==Further Reading==
	* [http://www.purdue.edu/newsroom/research/2011/110125ZiaiePaper.html Disposable microfluidic devices created using regular wax paper]
	* [http://www.rsc.org/Publishing/ChemTech/Volume/2008/01/Shrinky-Dink_microfluidics.asp Shrinky Dink® microfluidics] - academic paper [http://shrink.eng.uci.edu/papers/2008_Grimes.pdf here]
	* Wikipedia: [http://en.wikipedia.org/wiki/Microfluidics Microfluidics] and [http://en.wikipedia.org/wiki/Lab_on_a_chip Lab-on-a-chip]		* Wikipedia: [http://en.wikipedia.org/wiki/Microfluidics Microfluidics] and [http://en.wikipedia.org/wiki/Lab_on_a_chip Lab-on-a-chip]
	* [http://diybio.org/ DIY Bio]		* [http://diybio.org/ DIY Bio]
	* [http://www.dfa.org/ Diagnostics For All]		* [http://www.dfa.org/ Diagnostics For All]
	* [http://www.daktaridx.com/ Daktari]		* [http://www.daktaridx.com/ Daktari]

Conor at 00:42, 22 February 2011

2011-02-22T00:42:19Z

@@ Line 3: / Line 3: @@
 [[File:Microfluidic01.jpg|400px|thumb|right|Microfluidic Chip]]
-Analytical devices can be produced at very low cost from simple materials. Applications are as vast as they are revolutionary, and include medical tests, environmental sensing, agriculture, soil science and many more. [http://en.wikipedia.org/wiki/Microfluidics Microfluidics] refers to a set of technologies that control the flow of minute amounts of liquids or gases — typically measured in nano- and picoliters — in a miniaturized system. With these "chips", room-sized diagnostic testing equipment can be shrunk down to the size of a postage stamp. This is also called "lab-on-a-chip".
+[http://en.wikipedia.org/wiki/Microfluidics Microfluidics] refers to a set of technologies that control the flow of minute amounts of liquids or gases — typically measured in nano- and picoliters — in a miniaturized system.
 ==Materials and Equipment Used==
@@ Line 22: / Line 36: @@
 *plastic lenses for cheap microscopes
 *[[Laser cutter]], see [http://diyhpl.us/~bryan/papers/Low-cost%20rapid%20prototyping%20of%20flexible%20microfluidic%20devices%20using%20a%20desktop%20digital%20cutter.pdf Low-cost rapid prototyping of flexible microfluidic devices using a desktop digital craft cutter]
 ==George Whitesides, Harvard University==

Rasmus at 20:45, 21 February 2011

2011-02-21T20:45:01Z

← Older revision		Revision as of 20:45, 21 February 2011
Line 43:		Line 43:
	* Wikipedia: [http://en.wikipedia.org/wiki/Microfluidics Microfluidics] and [http://en.wikipedia.org/wiki/Lab_on_a_chip Lab-on-a-chip]		* Wikipedia: [http://en.wikipedia.org/wiki/Microfluidics Microfluidics] and [http://en.wikipedia.org/wiki/Lab_on_a_chip Lab-on-a-chip]
	* [http://diybio.org/ DIY Bio]		* [http://diybio.org/ DIY Bio]
			* [http://www.dfa.org/ Diagnostics For All]
			* [http://www.daktaridx.com/ Daktari]