Antibodies: Difference between revisions
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=Thoughts= | |||
*Antibodies are blood proteins that kill cellular intruders (disease) - [https://en.wikipedia.org/wiki/Antibody] | *Antibodies are blood proteins that kill cellular intruders (disease) - [https://en.wikipedia.org/wiki/Antibody] | ||
*How do you culture antibodies? Via hybridomas - [https://en.wikipedia.org/wiki/Hybridoma_technology] | *How do you culture antibodies? Via hybridomas - [https://en.wikipedia.org/wiki/Hybridoma_technology] | ||
*'''Once a hybridoma is produced, say for the COVID spike protein - then is mass production of it distributatable via basic DIY bio?''' | *'''Once a hybridoma is produced, say for the COVID spike protein - then is mass production of it distributatable via basic DIY bio?''' | ||
*Are monoclonal antibodies safe? They naturally have to be tested, just like anything else. [https://www.ncbi.nlm.nih.gov/pubmed/20615177] | *Are monoclonal antibodies safe? They naturally have to be tested, just like anything else. [https://www.ncbi.nlm.nih.gov/pubmed/20615177] | ||
*Once a hybridoma is available, does it produce reliable results? There is a process - [https://www.researchgate.net/post/How_should_I_culture_hybridoma_cells_for_antibody_collection] | |||
=Companies Working on It= | =Companies Working on It= | ||
*GigaGen - [https://www.fiercebiotech.com/biotech/gigagen-jumps-into-covid-19-arena-polyclonal-antibodies] - ''uses single-cell sequencing to “capture and recreate” whole libraries of antibodies from recovered COVID-19 patients. The company can then choose which of those antibodies to turn into recombinant polyclonal antibody treatments'' | *GigaGen - [https://www.fiercebiotech.com/biotech/gigagen-jumps-into-covid-19-arena-polyclonal-antibodies] - ''uses single-cell sequencing to “capture and recreate” whole libraries of antibodies from recovered COVID-19 patients. The company can then choose which of those antibodies to turn into recombinant polyclonal antibody treatments'' | ||
Revision as of 02:52, 6 April 2020
Thoughts
- Antibodies are blood proteins that kill cellular intruders (disease) - [1]
- How do you culture antibodies? Via hybridomas - [2]
- Once a hybridoma is produced, say for the COVID spike protein - then is mass production of it distributatable via basic DIY bio?
- Are monoclonal antibodies safe? They naturally have to be tested, just like anything else. [3]
- Once a hybridoma is available, does it produce reliable results? There is a process - [4]
Companies Working on It
- GigaGen - [5] - uses single-cell sequencing to “capture and recreate” whole libraries of antibodies from recovered COVID-19 patients. The company can then choose which of those antibodies to turn into recombinant polyclonal antibody treatments
- Regeneron - All coronaviruses have a cell surface protein called the spike protein. It helps the virus binds to the host cell for an infection. Regeneron’s SARS-CoV-2 antibodies will target the spike protein in an attempt to block the interaction of the virus with the host. - [6]
Definitions
- Antigen. [7] - ex: a virus.
- Recombinant DNA - genetic engineering of DNA that does not yet exist in nature - [8]
- Recombinant antibodies - [ecombinant antibodies are produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains into high-yield expression vectors - [9]
- Serological (antibody) vs PCR tests for COVID - [10]