Antibodies: Difference between revisions
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*Recombinant antibodies - [''ecombinant antibodies are produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains into high-yield expression vectors'' - [https://www.abcam.com/primary-antibodies/recombinant-antibodies] | *Recombinant antibodies - [''ecombinant antibodies are produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains into high-yield expression vectors'' - [https://www.abcam.com/primary-antibodies/recombinant-antibodies] | ||
*Serological (antibody) vs PCR tests for COVID - [https://www.healthline.com/health-news/how-antibody-testing-can-help-us-fight-covid-19#What-is-an-immunity-test?] | *Serological (antibody) vs PCR tests for COVID - [https://www.healthline.com/health-news/how-antibody-testing-can-help-us-fight-covid-19#What-is-an-immunity-test?] | ||
*Convalescent serum - [https://www.merriam-webster.com/dictionary/convalescent%20serum] | *Convalescent serum - antibody-rich serum from blood of recovered patients - [https://www.merriam-webster.com/dictionary/convalescent%20serum] | ||
Revision as of 02:54, 6 April 2020
Thoughts
- Antibodies are blood proteins that kill cellular intruders (disease) - [1]
- How do you culture antibodies? Via hybridomas - [2]
- Once a hybridoma is produced, say for the COVID spike protein - then is mass production of it distributatable via basic DIY bio?
- Are monoclonal antibodies safe? They naturally have to be tested, just like anything else. [3]
- Once a hybridoma is available, does it produce reliable results? There is a process - [4]
Companies Working on It
- GigaGen - [5] - uses single-cell sequencing to “capture and recreate” whole libraries of antibodies from recovered COVID-19 patients. The company can then choose which of those antibodies to turn into recombinant polyclonal antibody treatments
- Regeneron - All coronaviruses have a cell surface protein called the spike protein. It helps the virus binds to the host cell for an infection. Regeneron’s SARS-CoV-2 antibodies will target the spike protein in an attempt to block the interaction of the virus with the host. - [6]
Definitions
- Antigen. [7] - ex: a virus.
- Recombinant DNA - genetic engineering of DNA that does not yet exist in nature - [8]
- Recombinant antibodies - [ecombinant antibodies are produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains into high-yield expression vectors - [9]
- Serological (antibody) vs PCR tests for COVID - [10]
- Convalescent serum - antibody-rich serum from blood of recovered patients - [11]