PCR Test for COVID: Difference between revisions
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*SYBR Green (as in article above) Master Mix is $3/test - https://www.thermofisher.com/order/catalog/product/4344463#/4344463 | *SYBR Green (as in article above) Master Mix is $3/test - https://www.thermofisher.com/order/catalog/product/4344463#/4344463 | ||
*TRIzol RNA prep kit - [https://www.thermofisher.com/order/catalog/product/12183555#/12183555] | *TRIzol RNA prep kit - [https://www.thermofisher.com/order/catalog/product/12183555#/12183555] | ||
*Probes and primers are 23 cents each - source - [https://www.biosearchtech.com/products/pcr-kits-and-reagents/pathogen-detection/2019-ncov-cdc-probe-and-primer-kit-for-sars-cov-2] | |||
==Other Generic== | ==Other Generic== | ||
*PCR master mix - dNTPs, enzymes included - https://www.promega.com/-/media/files/resources/protocols/product-information-sheets/g/pcr-master-mix-protocol.pdf?la=en. | *PCR master mix - dNTPs, enzymes included - https://www.promega.com/-/media/files/resources/protocols/product-information-sheets/g/pcr-master-mix-protocol.pdf?la=en. | ||
*Cost for Master Mix is 40 cents - [https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/end-point-pcr/taq-pcr-master-mix-kit/#orderinginformation] | *Cost for Master Mix is 40 cents - [https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/end-point-pcr/taq-pcr-master-mix-kit/#orderinginformation] | ||
* | |||
=What Gives?= | |||
*''The antibody test, a rapid diagnostic developed by Abbott Laboratories that requires a simple, single finger prick, takes five minutes to complete and cost Egyptians a mere 50¢. Genome tests developed by Roche, which were administered to nearly four million, cost no more than $5 each. That’s a sharp contrast to what people are charging today to test for COVID-19—in some cases $10 to $20 for a serology test, and more than $100 for a genome test.'' - [https://www.forbes.com/sites/williamhaseltine/2020/04/08/pricing-and-profiteering-from-covid-19-tests/#41d05ec51ccb] | |||
*Roche test - [https://diagnostics.roche.com/us/en/products/params/cobas-sars-cov-2-test.html] | |||
==Difficulties== | |||
* Controls - positive control of covid sequence | |||
* verification of RNA extraction to prevent false negative | |||
* primer verification to prevent false positives | |||
Latest revision as of 00:19, 14 April 2020
General
https://jamanetwork.com/journals/jama/fullarticle/2764238
Low Cost Test
- Low Cost and Safe COVID Detection
- $15 and 4 hours - [$15 (without taking an account of labor cost) per testing, while the total duration of the protocol was found to be approximately 4 hours. ]
Sourcing
- SYBR Green (as in article above) Master Mix is $3/test - https://www.thermofisher.com/order/catalog/product/4344463#/4344463
- TRIzol RNA prep kit - [1]
- Probes and primers are 23 cents each - source - [2]
Other Generic
- PCR master mix - dNTPs, enzymes included - https://www.promega.com/-/media/files/resources/protocols/product-information-sheets/g/pcr-master-mix-protocol.pdf?la=en.
- Cost for Master Mix is 40 cents - [3]
What Gives?
- The antibody test, a rapid diagnostic developed by Abbott Laboratories that requires a simple, single finger prick, takes five minutes to complete and cost Egyptians a mere 50¢. Genome tests developed by Roche, which were administered to nearly four million, cost no more than $5 each. That’s a sharp contrast to what people are charging today to test for COVID-19—in some cases $10 to $20 for a serology test, and more than $100 for a genome test. - [4]
- Roche test - [5]
Difficulties
- Controls - positive control of covid sequence
- verification of RNA extraction to prevent false negative
- primer verification to prevent false positives