Thin Layer Chromatography: Difference between revisions
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=Setups= | =Setups= | ||
==Low Budget== | ==Low Budget== | ||
*Flat Bottom Glass Beaker | *Flat Bottom [[Glass Beaker]] | ||
*and/or a Glass TLC Chamber | *and/or a Glass TLC Chamber (Such as [https://a.co/d/5Aq8zUS This 10cm Model by StonyLab] ) | ||
*A [[Dropper]] or better yet a [[Pipette]] / [[Micropipette]] | *A [[Dropper]] or better yet a [[Pipette]] / [[Micropipette]] | ||
*Solvents | *Appropriate Solvents and Stains | ||
==Medium Budget== | ==Medium Budget== | ||
| Line 25: | Line 24: | ||
==High End== | ==High End== | ||
*Medium, but automated sampling / [[Mass Spectrometry]] etc is integrated | *Medium, but automated sampling / [[Mass Spectrometry]] etc is integrated | ||
=Consumables= | |||
==Paper== | |||
*[[Printer Paper]] | |||
*Are Specialized Papers Available? | |||
==Plates== | |||
===Cellulosic=== | |||
*[https://www.sorbtech.com/chromatography/thin-layer-chromatography-tlc-plates/specialty-plates/cellulose-tlc-plates/ Sorbtech Cellulose TLC Plates] (Some are [[PEI]] Blends) | |||
===Silica=== | |||
*[https://www.fishersci.com/us/en/browse/90126184/tlc-plates?page=1 Fischer Scientific’s Products] | |||
=Internal Links= | =Internal Links= | ||
* | *[[Saturate, Aromatic, Resin and Asphaltene Fractionation]] | ||
**TLC with some form of sensing can be used for this, and many of the papers describe this process | |||
=External Links= | =External Links= | ||
*[https://en.m.wikipedia.org/wiki/Thin-layer_chromatography The Wikipedia Page on Thin Layer Chromatography] | *[https://en.m.wikipedia.org/wiki/Thin-layer_chromatography The Wikipedia Page on Thin Layer Chromatography] | ||
*[https://www.sciencedirect.com/science/article/abs/pii/S0016236108001695 A 2008 Study in the Journal “Fuel” Titled “ Thin layer chromatography and image analysis to detect glycerol in biodiesel” ] | *Two [[Biodiesel]] Related Papers: | ||
**[https://pubmed.ncbi.nlm.nih.gov/21316691/ A 2011 Paper Titled “ Analysis of biodiesel conversion using thin layer chromatography and nonlinear calibration curves” ] | |||
**[https://www.sciencedirect.com/science/article/abs/pii/S0016236108001695 A 2008 Study in the Journal “Fuel” Titled “ Thin layer chromatography and image analysis to detect glycerol in biodiesel” ] | |||
*Essential Oil Related Papers: | |||
**[https://www.researchgate.net/publication/342869656_Thin-layer_Chromatography_Analysis_of_Nigella_Sativa_L_Essential_Oil A 2020 Study in “ Journal of Hygienic Engineering and Design” Titled “ Thin-layer Chromatography Analysis of Nigella Sativa L. Essential Oil” ] | |||
**[https://pubmed.ncbi.nlm.nih.gov/37698178/ A 2023 Paper Titled “ A high performance thin layer chromatography (HPTLC) method for the quality assessment of citronella oil: application in commercial sample analysis” ] | |||
*[https://www.researchgate.net/publication/269875440_Identifying_Type_of_Refined_Petroleum_Products_in_Environmental_Media_Thin-Layer_Chromatography_TLC_as_a_Quick_Methodology A 2014 Study in the Journal “Water Air and Soil Pollution” Titled “Identifying Type of Refined Petroleum Products in Environmental Media: Thin-Layer Chromatography (TLC) as a Quick Methodology” ] | *[https://www.researchgate.net/publication/269875440_Identifying_Type_of_Refined_Petroleum_Products_in_Environmental_Media_Thin-Layer_Chromatography_TLC_as_a_Quick_Methodology A 2014 Study in the Journal “Water Air and Soil Pollution” Titled “Identifying Type of Refined Petroleum Products in Environmental Media: Thin-Layer Chromatography (TLC) as a Quick Methodology” ] | ||
*[https://academic.oup.com/chromsci/article/36/10/487/297611 An Article in “The Journal of Chromatographic Science” Titled “ Suitability of Thin-Layer Chromatography-Flame Ionization Detection with Regard to Quantitative Characterization of Different Fossil Fuel Products. II. Calibration Methods Concerning Quantitative Hydrocarbon-Group Type Analysis” ] | |||
*[https://pubs.acs.org/doi/10.1021/acs.energyfuels.8b00639 A 2018 Article in the Journal “Energy and Fuels” Titled “ Evaluation of Thin-Layer Chromatography–Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Imaging for Visualization of Crude Oil Interactions” ] | |||
*[https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/rcm.9409 A 2022 Paper Titled “ Thin layer chromatography/desorption flame-induced atmospheric pressure chemical ionization/mass spectrometry for the analysis of volatile and semi-volatile mixtures” ] | |||
*[https://pubs.acs.org/doi/abs/10.1021/ac901514c A 2009 Paper Titled “ Thin-Layer Chromatography/Laser-Induced Acoustic Desorption/Electrospray Ionization Mass Spectrometry” ] | |||
[[Category: Bio-Petrochemistry]] [[Category: Lab Equipment]] | |||
Latest revision as of 21:21, 27 September 2024
Basics
- A form of Chromatography using a “mobile phase” which spreads the “stationary phase” (the sample) over a piece of material (either a “TLC Plate” or (TLC) Paper)
- Useful not just for research, but also for Quality Control of materials
- Simple Setups can be VERY accessible ( Printer Paper , a Glass Jar, a Dropper, and a Solvent)
- High End Setups can be fully automated and produce Quantitative Data
OSE Use Cases
- Chemical Synthesis Purity Testing
- Mainly in Regards to Bio-Petrochemistry Lab Scale Chemistry
- This can also be done to see if a reaction step has “completed”
- An example is This Video the YouTube Channel “Wheeler Scientific” Titled “From Lab to Backyard: How is DEET in Bug Spray Made? (Specifically at the timecode 5:33 )
- Routine Quality Control use
Setups
Low Budget
- Flat Bottom Glass Beaker
- and/or a Glass TLC Chamber (Such as This 10cm Model by StonyLab )
- A Dropper or better yet a Pipette / Micropipette
- Appropriate Solvents and Stains
Medium Budget
- A Lab Bot for automating the process and a scanner/camera setup to record the results
High End
- Medium, but automated sampling / Mass Spectrometry etc is integrated
Consumables
Paper
- Printer Paper
- Are Specialized Papers Available?
Plates
Cellulosic
- Sorbtech Cellulose TLC Plates (Some are PEI Blends)
Silica
Internal Links
- Saturate, Aromatic, Resin and Asphaltene Fractionation
- TLC with some form of sensing can be used for this, and many of the papers describe this process
External Links
- The Wikipedia Page on Thin Layer Chromatography
- Two Biodiesel Related Papers:
- Essential Oil Related Papers:
- A 2020 Study in “ Journal of Hygienic Engineering and Design” Titled “ Thin-layer Chromatography Analysis of Nigella Sativa L. Essential Oil”
- A 2023 Paper Titled “ A high performance thin layer chromatography (HPTLC) method for the quality assessment of citronella oil: application in commercial sample analysis”
- A 2014 Study in the Journal “Water Air and Soil Pollution” Titled “Identifying Type of Refined Petroleum Products in Environmental Media: Thin-Layer Chromatography (TLC) as a Quick Methodology”
- An Article in “The Journal of Chromatographic Science” Titled “ Suitability of Thin-Layer Chromatography-Flame Ionization Detection with Regard to Quantitative Characterization of Different Fossil Fuel Products. II. Calibration Methods Concerning Quantitative Hydrocarbon-Group Type Analysis”
- A 2018 Article in the Journal “Energy and Fuels” Titled “ Evaluation of Thin-Layer Chromatography–Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Imaging for Visualization of Crude Oil Interactions”
- A 2022 Paper Titled “ Thin layer chromatography/desorption flame-induced atmospheric pressure chemical ionization/mass spectrometry for the analysis of volatile and semi-volatile mixtures”
- A 2009 Paper Titled “ Thin-Layer Chromatography/Laser-Induced Acoustic Desorption/Electrospray Ionization Mass Spectrometry”