Thin Layer Chromatography: Difference between revisions
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(Added some more links under the “External Links” section) |
(Added some more links under the “External Links” section) |
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*[https://en.m.wikipedia.org/wiki/Thin-layer_chromatography The Wikipedia Page on Thin Layer Chromatography] | *[https://en.m.wikipedia.org/wiki/Thin-layer_chromatography The Wikipedia Page on Thin Layer Chromatography] | ||
*[https://www.sciencedirect.com/science/article/abs/pii/S0016236108001695 A 2008 Study in the Journal “Fuel” Titled “ Thin layer chromatography and image analysis to detect glycerol in biodiesel” ] | *[https://www.sciencedirect.com/science/article/abs/pii/S0016236108001695 A 2008 Study in the Journal “Fuel” Titled “ Thin layer chromatography and image analysis to detect glycerol in biodiesel” ] | ||
*[https://www.researchgate.net/publication/269875440_Identifying_Type_of_Refined_Petroleum_Products_in_Environmental_Media_Thin-Layer_Chromatography_TLC_as_a_Quick_Methodology A 2014 Study in the Journal “Water Air and Soil Pollution” Titled “Identifying Type of Refined Petroleum Products in Environmental Media: Thin-Layer Chromatography (TLC) as a Quick Methodology” ] | |||
Revision as of 20:30, 27 September 2024
Basics
- A form of Chromatography using a “mobile phase” which spreads the “stationary phase” (the sample) over a piece of material (either a “TLC Plate” or (TLC) Paper)
- Useful not just for research, but also for Quality Control of materials
- Simple Setups can be VERY accessible ( Printer Paper , a Glass Jar, a Dropper, and a Solvent)
- High End Setups can be fully automated and produce Quantitative Data
OSE Use Cases
- Chemical Synthesis Purity Testing
- Mainly in Regards to Bio-Petrochemistry Lab Scale Chemistry
- This can also be done to see if a reaction step has “completed”
- An example is This Video the YouTube Channel “Wheeler Scientific” Titled “From Lab to Backyard: How is DEET in Bug Spray Made? (Specifically at the timecode 5:33 )
- Routine Quality Control use
Setups
Low Budget
- Flat Bottom Glass Beaker
- and/or a Glass TLC Chamber
- A Dropper or better yet a Pipette / Micropipette
- Solvents
- Stains can help too
Medium Budget
- A Lab Bot for automating the process and a scanner/camera setup to record the results
High End
- Medium, but automated sampling / Mass Spectrometry etc is integrated
Internal Links
External Links
- The Wikipedia Page on Thin Layer Chromatography
- A 2008 Study in the Journal “Fuel” Titled “ Thin layer chromatography and image analysis to detect glycerol in biodiesel”
- A 2014 Study in the Journal “Water Air and Soil Pollution” Titled “Identifying Type of Refined Petroleum Products in Environmental Media: Thin-Layer Chromatography (TLC) as a Quick Methodology”