Thin Layer Chromatography: Difference between revisions
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*[https://academic.oup.com/chromsci/article/36/10/487/297611 An Article in “The Journal of Chromatographic Science” Titled “ Suitability of Thin-Layer Chromatography-Flame Ionization Detection with Regard to Quantitative Characterization of Different Fossil Fuel Products. II. Calibration Methods Concerning Quantitative Hydrocarbon-Group Type Analysis” ] | *[https://academic.oup.com/chromsci/article/36/10/487/297611 An Article in “The Journal of Chromatographic Science” Titled “ Suitability of Thin-Layer Chromatography-Flame Ionization Detection with Regard to Quantitative Characterization of Different Fossil Fuel Products. II. Calibration Methods Concerning Quantitative Hydrocarbon-Group Type Analysis” ] | ||
*[https://pubs.acs.org/doi/10.1021/acs.energyfuels.8b00639 A 2018 Article in the Journal “Energy and Fuels” Titled “ Evaluation of Thin-Layer Chromatography–Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Imaging for Visualization of Crude Oil Interactions” ] | *[https://pubs.acs.org/doi/10.1021/acs.energyfuels.8b00639 A 2018 Article in the Journal “Energy and Fuels” Titled “ Evaluation of Thin-Layer Chromatography–Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Imaging for Visualization of Crude Oil Interactions” ] | ||
*[https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/rcm.9409 A 2022 Paper Titled “ Thin layer chromatography/desorption flame-induced atmospheric pressure chemical ionization/mass spectrometry for the analysis of volatile and semi-volatile mixtures” ] | |||
Revision as of 20:57, 27 September 2024
Basics
- A form of Chromatography using a “mobile phase” which spreads the “stationary phase” (the sample) over a piece of material (either a “TLC Plate” or (TLC) Paper)
- Useful not just for research, but also for Quality Control of materials
- Simple Setups can be VERY accessible ( Printer Paper , a Glass Jar, a Dropper, and a Solvent)
- High End Setups can be fully automated and produce Quantitative Data
OSE Use Cases
- Chemical Synthesis Purity Testing
- Mainly in Regards to Bio-Petrochemistry Lab Scale Chemistry
- This can also be done to see if a reaction step has “completed”
- An example is This Video the YouTube Channel “Wheeler Scientific” Titled “From Lab to Backyard: How is DEET in Bug Spray Made? (Specifically at the timecode 5:33 )
- Routine Quality Control use
Setups
Low Budget
- Flat Bottom Glass Beaker
- and/or a Glass TLC Chamber
- A Dropper or better yet a Pipette / Micropipette
- Solvents
- Stains can help too
Medium Budget
- A Lab Bot for automating the process and a scanner/camera setup to record the results
High End
- Medium, but automated sampling / Mass Spectrometry etc is integrated
Consumables
Paper
- Printer Paper
- Are Specialized Papers Available?
Plates
Cellulosic
- Sorbtech Cellulose TLC Plates (Some are PEI Blends)
Silica
Internal Links
External Links
- The Wikipedia Page on Thin Layer Chromatography
- Two Biodiesel Related Papers:
- A 2011 Paper Titled “ Analysis of biodiesel conversion using thin layer chromatography and nonlinear calibration curves”
- A 2008 Study in the Journal “Fuel” Titled “ Thin layer chromatography and image analysis to detect glycerol in biodiesel”
- A 2014 Study in the Journal “Water Air and Soil Pollution” Titled “Identifying Type of Refined Petroleum Products in Environmental Media: Thin-Layer Chromatography (TLC) as a Quick Methodology”
- An Article in “The Journal of Chromatographic Science” Titled “ Suitability of Thin-Layer Chromatography-Flame Ionization Detection with Regard to Quantitative Characterization of Different Fossil Fuel Products. II. Calibration Methods Concerning Quantitative Hydrocarbon-Group Type Analysis”
- A 2018 Article in the Journal “Energy and Fuels” Titled “ Evaluation of Thin-Layer Chromatography–Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Imaging for Visualization of Crude Oil Interactions”
- A 2022 Paper Titled “ Thin layer chromatography/desorption flame-induced atmospheric pressure chemical ionization/mass spectrometry for the analysis of volatile and semi-volatile mixtures”