Fermentor/Research Development: Difference between revisions
No edit summary |
|||
| Line 1: | Line 1: | ||
=Fermentor design= | =Fermentor design= | ||
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1058040/pdf/applmicro00350-0046.pdf | Dialysis Fermentor Systems for Concentrated Culture of Microorganisms] by Gallup and Gerhardt (1963) describes a fermentor connected to a dialysis unit that allowed extremely high cell density and other favorable features. The semipermeable membrane was made of regenerated [[cellulose]] and held taut in a frame in a separate chamber.. Feeding could be conducted by exchange at the membrane. Extremely high cell density was achieved with the ''Serratia marcescens'' culture. When a reservoir chamber was used to with dialysis tubing a second phase to the growth phase was introduced. Addition of polyethylene glycol can drive the water from a culture and cause them to flocculate (and can clog a machine). | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1058040/pdf/applmicro00350-0046.pdf | Dialysis Fermentor Systems for Concentrated Culture of Microorganisms] by Gallup and Gerhardt (1963) describes a fermentor connected to a dialysis unit that allowed extremely high cell density and other favorable features. The semipermeable membrane was made of regenerated [[cellulose]] and held taut in a frame in a separate chamber.. Feeding could be conducted by exchange at the membrane. Extremely high cell density was achieved with the ''Serratia marcescens'' culture. When a reservoir chamber was used to with dialysis tubing a second phase to the growth phase was introduced. Addition of polyethylene glycol can drive the water from a culture and cause them to flocculate (and can clog a machine). | ||
Fermentor for E. coli protein expression: <br/> | |||
http://www.biotechniques.com/BiotechniquesJournal/2008/August/An-automated-home-built-low-cost-fermenter-suitable-for-large-scale-bacterial-expression-of-proteins-in-Escherichia-coli/biotechniques-45258.html | |||
Supplementary contains build instructions and bill of materials: http://www.biotechniques.com/multimedia/archive/00041/318_41920a.pdf | |||
http://www.coe.neu.edu/Depts/test/MIE/pdfs/Capstone/Mechanical_Engineering/ME%202010%2012%20Bioreactor%20to%20Enable%20Microbe%20Productivity%20Optimiz.pdf | |||
https://biogas.ifas.ufl.edu/Publs/BioresourTechnol-92(1)-2004.pdf | |||
http://www.sky.cz/lambda/pdf/bioreactors-article.pdf | http://www.sky.cz/lambda/pdf/bioreactors-article.pdf | ||
Revision as of 20:02, 16 July 2012
Fermentor design
| Dialysis Fermentor Systems for Concentrated Culture of Microorganisms by Gallup and Gerhardt (1963) describes a fermentor connected to a dialysis unit that allowed extremely high cell density and other favorable features. The semipermeable membrane was made of regenerated cellulose and held taut in a frame in a separate chamber.. Feeding could be conducted by exchange at the membrane. Extremely high cell density was achieved with the Serratia marcescens culture. When a reservoir chamber was used to with dialysis tubing a second phase to the growth phase was introduced. Addition of polyethylene glycol can drive the water from a culture and cause them to flocculate (and can clog a machine).
Fermentor for E. coli protein expression:
http://www.biotechniques.com/BiotechniquesJournal/2008/August/An-automated-home-built-low-cost-fermenter-suitable-for-large-scale-bacterial-expression-of-proteins-in-Escherichia-coli/biotechniques-45258.html
Supplementary contains build instructions and bill of materials: http://www.biotechniques.com/multimedia/archive/00041/318_41920a.pdf
https://biogas.ifas.ufl.edu/Publs/BioresourTechnol-92(1)-2004.pdf
http://www.sky.cz/lambda/pdf/bioreactors-article.pdf
http://pac.iupac.org/publications/pac/pdf/1973/pdf/3603x0305.pdf
http://chem.engr.utc.edu/ench435/2004/FromTablet/FILTER-AIDS.pdf
Blogged fermentor builds
http://benkrasnow.blogspot.com/2009/09/stainless-steel-conical-beer-fermentor.html http://hbd.org/brewniversity/engineering/conical/ http://nilrodbrewery.blogspot.com/2010/12/diy-inductor-tank-conical-fermenter.html